Part:BBa_K4341004:Experience

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Experience by 2022 iGEM OhioState

Inspired by OhioState 2021 we fused BBa_K434001 to BBa_K4341000 in an attempt to create a very strong promoter for use in our Phinder Phage. We decided to create a fusion of mCherry to BBa_K4341004 to determine its expression profile. Once the fusion of mCherry and BBa_K4341004 was obtained we attempted to ligate into the pET28a backbone (given to us by Dr. David Wood's Lab). However once isolation via gel extraction was done for our backbone and inserts were digested with BamHI and NotI ligation transformations proved fruitless after many attempts and swapping of buffers. Eventually because of time constraints were unable to pursue the problem any further.

Figure 1. Gel Electrophoresis Result: Lane 1 rrnB P1; Lane 2 pR; Lane 3 pR-rrnB P1; Lane 4 T5-rrnB P1

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